Secondary Antibodies
Secondary antibodies bind to a primary antibody to assist in detection, amplification, sorting, and purification of target antigens, and are used in various biochemical techniques/assays (e.g., ELISA, western blot (WB), immunohistochemistry).
Goat Secondary Antibodies
(44,182)
Mouse Secondary Antibodies
(14,490)
Rabbit Secondary Antibodies
(6,302)
Donkey Secondary Antibodies
(4,319)
Rat Secondary Antibodies
(1,234)
Sheep Secondary Antibodies
(701)
Chicken Secondary Antibodies
(403)
Bovine Secondary Antibodies
(257)
Pig Secondary Antibodies
(76)
Horse Secondary Antibodies
(74)
Alpaca Secondary Antibodies
(62)
Hamster Secondary Antibodies
(57)
Biosimilar Secondary Antibodies
(9)
Human Secondary Antibodies
(7)
Duck Secondary Antibodies
(3)
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Goat anti-Golden Syrian Hamster IgG (H+L) Secondary Antibody, HRP, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Golden Syrian Hamster |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000 - 1:5000, ELISA 1:7000 - 1:35000, Immunohistochemistry 1:500 - 1:2500 |
| Classification | Polyclonal |
| Immunogen | Goat anti-Golden Syrian Hamster IgG (H+L) Secondary Antibody [HRP] was produced by repeated immunization with Golden Syrian Hamster IgG whole molecule in goat. |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | Diminished reactivity will occur against Armenian Hamster IgG. |
Goat anti-Rat IgG Fc Secondary Antibody, Texas Red, Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Rat |
| Host Species | Goat |
| Conjugate | Texas Red |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000 - 1:10000, Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Rat IgG F(c) fragment |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Rat IgG F(ab). |
Donkey anti-Sheep IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Donkey Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Sheep |
| Host Species | Donkey |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000 - 1:10000, ELISA 1:65000, Immunohistochemistry 1:500 - 1:2500 |
| Classification | Polyclonal |
| Immunogen | Donkey anti-Sheep IgG (H+L) Secondary Antibody [HRP] (Pre-adsorbed) was produced by repeated immunization with Sheep IgG whole molecule in donkey. |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, and Rat Serum Proteins. No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit and Rat Serum Proteins. |
Goat anti-Chicken IgG (H+L) Secondary Antibody, DyLight 680 (Pre-adsorbed), Novus Biologicals™
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Chicken |
| Host Species | Goat |
| Conjugate | DyLight 680 |
| Applications | Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence,Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:10000, Immunocytochemistry/Immunofluorescence 1:5000, Fluorophore-linked immunosorbent assay 1:20000 |
| Classification | Polyclonal |
| Immunogen | Chicken IgG whole molecule |
| Reconstitution | Reconstitute with 100 ul deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, and Sheep Serum Proteins. No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins. |
Rabbit anti-Canine IgG Fc Secondary Antibody, Rhodamine, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Canine |
| Host Species | Rabbit |
| Conjugate | Rhodamine |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG Fc |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Dog IgG F(c) fragment |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Dog IgG F(ab'). |
Rabbit anti-Human IgA Heavy Chain Secondary Antibody, HRP, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgA |
| Concentration | LYOPH |
| Antigen | IgA Heavy Chain |
| Gene Symbols | IGHA1 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:1000 - 1:5000, ELISA 1:56000, Immunohistochemistry 1:500 - 1:2500 |
| Gene Alias | Immunoglobulin A |
| Gene ID (Entrez) | 3493 |
| Classification | Polyclonal |
| Immunogen | Human IgA a alpha chain |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | Specificity was confirmed by ELISA minimal cross reactivity against other Human heavy or light chain isotypes. |
Rabbit anti-Mouse IgG1 Heavy Chain Secondary Antibody, HRP, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Rabbit |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG1 |
| Concentration | LYOPH |
| Antigen | IgG1 Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:20000, ELISA 1:20000 - 1:200000, Immunohistochemistry 1:500 - 1:2500 |
| Classification | Polyclonal |
| Immunogen | Mouse IgG1 heavy chain |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | Specificity was confirmed by ELISA against other mouse or human heavy chain isotypes. Cross reactivity with Mouse IgG2b and IgG3 is 1%. |
Rabbit anti-Human IgG (H+L) Secondary Antibody, FITC (Pre-adsorbed), Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C in the dark. |
|---|---|
| Target Species | Human |
| Host Species | Rabbit |
| Conjugate | Fluorescein |
| Applications | Flow Cytometry,Fluorophore-Linked Immunosorbent Assay,Immunocytochemistry/Immunofluorescence |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Flow Cytometry 1:1:10 - 1:1000, Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000, Fluorophore-linked immunosorbent assay 1:10000 - 1:50000 |
| Classification | Polyclonal |
| Immunogen | Human IgG whole molecule |
| Reconstitution | Reconstitute with 1.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | This antibody was pre-adsorbed against Mouse Serum Proteins. No reaction was observed against Mouse Serum Proteins. |
Rabbit anti-Bovine IgG F(ab')2 Secondary Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Bovine |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Antigen | IgG F(ab')2 |
| Regulatory Status | RUO |
| Purification Method | Affinity Purified |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Bovine IgG F(ab')2 fragment |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Bovine IgG F(c). |
Rabbit anti-Equine IgG F(ab')2 Secondary Antibody, Novus Biologicals™
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Rabbit Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Aliquot reconstituted liquid and store at -20°C. Avoid freeze-thaw cycles. |
|---|---|
| Target Species | Equine |
| Host Species | Rabbit |
| Conjugate | Unconjugated |
| Applications | ELISA,Immunohistochemistry,Western Blot |
| Isotype | IgG |
| Concentration | LYOPH |
| Antigen | IgG F(ab')2 |
| Regulatory Status | RUO |
| Purification Method | Delipidation and Defibrination |
| Dilution | Western Blot 1:2000 - 1:10000, ELISA 1:20000 - 1:100000, Immunohistochemistry 1:1000 - 1:5000 |
| Gene Alias | immunoglobulin heavy constant gamma 1 (G1m marker) |
| Classification | Polyclonal |
| Immunogen | Horse IgG F(ab')2 fragment |
| Reconstitution | Reconstitute with 2.0 ml deionized water (or equivalent). |
| Primary or Secondary | Secondary |
| Test Specificity | No reaction was observed against Horse IgG F(c). |
Chicken anti-Mouse IgG (H+L) Secondary Antibody, Alkaline Phosphatase, Novus Biologicals™
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Chicken Polyclonal Antibody
| Content And Storage | Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Mouse |
| Host Species | Chicken |
| Conjugate | Alkaline Phosphatase (AP) |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | 1.50mg/mL |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Purified Mouse IgG (H&L) |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on mouse IgG and light chains on all mouse immunoglobulins |
Chicken anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 550, Novus Biologicals™
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Chicken Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Rabbit |
| Host Species | Chicken |
| Conjugate | DyLight 550 |
| Applications | Flow Cytometry,Immunohistochemistry,Immunomicroscopy |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Flow Cytometry 1:20 - 1:2000, Immunohistochemistry, Immunomicroscopy 1:20 - 1:2000 |
| Classification | Polyclonal |
| Immunogen | Purified Rabbit IgG (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins |
Goat F(ab')2 anti-Human IgG (H+L) Secondary Antibody, HRP (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | HRP |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:200-1:5000, ELISA 1:200-1:5000, Immunohistochemistry 1:500-1:5000, Immunohistochemistry-Paraffin |
| Classification | Polyclonal |
| Immunogen | Purified Human IgG (H&L) |
| Reconstitution | Rehydrate with 0.55 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on human IgG and light chains on all human immunoglobulins. This antibody has been pre-adsorbed against bovine, mouse, and rabbit serum proteins. |
Goat anti-Rabbit IgG (H+L) Secondary Antibody, DyLight 650 (Pre-adsorbed), Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store lyophilized antibody at 4°C. Mix reconstituted liquid with an equal volume of glycerol, aliquot contents and freeze at -20°C or below for long term storage. |
|---|---|
| Target Species | Rabbit |
| Host Species | Goat |
| Conjugate | DyLight 650 |
| Applications | Flow Cytometry,Immunomicroscopy |
| Isotype | IgG |
| Research Discipline | Adaptive Immunity, Immunology |
| Concentration | LYOPH |
| Antigen | IgG (H+L) |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Flow Cytometry 1:20 - 1:2000, Immunomicroscopy 1:20 - 1:2000 |
| Classification | Polyclonal |
| Immunogen | Purified Rabbit IgG (H&L) |
| Reconstitution | Rehydrate with 1.1 ml of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy gamma chains on rabbit IgG and light chains on all rabbit immunoglobulins. This antibody has been pre-adsorbed against bovine, goat, human, mouse, rat IgG and serum proteins. |
Goat F(ab')2 anti-Human IgM Heavy Chain Secondary Antibody, Biotin, Novus Biologicals™
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Goat Polyclonal Antibody
| Content And Storage | Store at 4C. After dilution, add an equal volume of glycerol to prevent loss of enzymatic activity and store at -20C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Target Species | Human |
| Host Species | Goat |
| Conjugate | Biotin |
| Applications | ELISA,Immunohistochemistry,Immunohistochemistry (Paraffin),Western Blot |
| Isotype | IgM |
| Concentration | 1.0mg/mL |
| Antigen | IgM Heavy Chain |
| Regulatory Status | RUO |
| Purification Method | Affinity purified |
| Dilution | Western Blot 1:2000 - 1:20000, ELISA 1:2000 - 1:20000, Immunohistochemistry 1:500 - 1:5000, Immunohistochemistry-Paraffin |
| Gene Alias | Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain |
| Gene ID (Entrez) | 3507 |
| Classification | Polyclonal |
| Immunogen | Purified Human IgM |
| Primary or Secondary | Secondary |
| Test Specificity | Based on IEP, this antibody reacts with heavy mu chains on human IgM |